ABSTRACT
BACKGROUND: In recent years, skin tissue engineering technology has been well-developed and plays increasingly important roles in dermatology studies. The development of a functional epidermal basement membrane, also called dermal-epidermal junction, is one of the most sought after aspects of skin tissue engineering,and one of the hardest to recreate in vitro.At the dermal-epidermal junction level,dermal fibroblasts and epidermal keratinocytes interact to form an interlinked basement membrane of extracellular matrix, which forms as a concerted action of both keratinocytes and fibroblasts. Successful formation of this basement membrane is essential for the stability of human skin. OBJECTIVE: To summarize the molecular organization and impact of epidermal basement membrane on skin homeostasis, and to review the applications of reconstructed human skin model in epidermal basement membrane studies, providing solutions for related dermato-pathological and physiological issues. METHODS: A computer-based online search was performed in PubMed database and Google Scholar with the defined publication date ranging from 2000 until September 2016. The keywords were "skin, basement membrane, dermal epidermal junction, skin equivalent, reconstructed skin, skin homeostasis". Finally 57 pertinent articles were screened out of the preliminary retrival list for further analysis. RESULTS AND CONCLUSION: Epidermal basement membrane is shown to play gate-keeping roles, which controls cellular behavior and diffusion of bioactive molecules via outside-in signaling.It is the foundation of epidermal integrity and skin homeostasis.The abnormal expression of basement membrane molecules will lead to skin physiological changes and even skin diseases.In vitro reconstructed skin model, highly resembling the normal human skin in structure and molecular organization, is becoming a very powerful tool to study the physiology of epidermal basement membrane, and also very promising for skin anti-aging research and the treatment of blistering diseases caused by epidermal basement membrane disorders.
ABSTRACT
Objective To develop the eye health related behaviors questionnaire for school age children and to evaluate its reliability and validity.Methods The initial questionnaire was formulated by literature review,preliminary-work outcomes and expert consultation.Totally 288 students in 4-5 grades from a elementary school in Wenzhou were selected.Two-independent samples t-test,pearson correlation coefficient analysis,test of internal consistency and factor analysis were conducted to examine the reliability and validity.Results Eye health related behaviors questionnaire for school age children was consisted of 28 items and 3 dimensions.Exploratory factor analysis suggested that 7 factors accounted for 63.76％ of the accumulated variances.The questionnaire-level content validity index was 0.89.The Cronbach's α coefficient was 0.82,and the spilt-half reliability coefficient was 0.844.The test-retest reliability coefficient was 0.682.Conclusion Eye health related behaviors questionnaire for school age children has good reliability and validity,which could be applied to measure children's eye health related behaviors.
ABSTRACT
Objective: To design and prepare siRNAs targeting ski gene and to observe its influence on the biological functions of HepG2 cells, such as proliferation, cell cycle, apoptosis, etc.. Methods: Three specific siRNAs of ski were designed and synthesized, and were transiently transfected into HepG2 via cathodolyte liposome transfection method. Real-time PCR and Western blotting were used to measure ski expression at mRNA and protein levels. The cell proliferation was assessed by MTT assay and the changes in cell cycle and apoptosis were evaluated by flow cytometry. Results: All the 3 specific ski-siRNA(A, B, C) effectively inhibited the expression of ski gene, with ski-siRNA-B having the highest inhibition rate(70%). Furthermore, the ski expression had a decreasing tendency with the transfection time. The proliferation of HepG2 cells was markedly inhibited by ski-siRNAs(P<0.05); the number of cells at S stage was obviously decreased, being 2 folds that of the negative control group. Conclusion: The siRNA of ski gene can effectively induce growth inhibition of HepG2 cells and reduce cells of S stage, which is possibly through down-regulation of ski gene.